WST-8 (Water-Soluble Tetrazolium 8) is a water-soluble tetrazolium salt widely used to assess the metabolic activity of cells. The dye does not penetrate living cells but can be reduced outside the cells by NADPH-dependent cellular oxidoreductases to water-soluble formazan. The reaction occurs by electron transfer across the plasma membrane in a neutral pH and the presence of an intermediate electron acceptor, 1-methoxyphenazine methosulfate. The staining intensity is proportional to the number of viable cells. The maximum absorption of the reaction product is 450–500 nm.
1. Fundamental Properties of the Product
Product Name: WST-8
Synonyms: 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2h-tetrazolium inner salt sodium salt (1:1);2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monoSodium salt;Cell Proliferation Assay;2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophen;GLT008;5-(2,4-Disulfophenyl)-3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazoliuM Inner Salt SodiuM Salt;5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium;Sodium 4-[2-(2-methoxy-4-nitro-phenyl)-3-(4-nitrophenyl)tetrazol-3-ium-5-yl]benzene-1,3-disulfonate
CAS: 193149-74-5
MF: C20H15N6NaO11S2
MW: 601.48
Synonyms: 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2h-tetrazolium inner salt sodium salt (1:1);2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium,monoSodium salt;Cell Proliferation Assay;2-(2-Methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophen;GLT008;5-(2,4-Disulfophenyl)-3-(2-Methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazoliuM Inner Salt SodiuM Salt;5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium;Sodium 4-[2-(2-methoxy-4-nitro-phenyl)-3-(4-nitrophenyl)tetrazol-3-ium-5-yl]benzene-1,3-disulfonate
CAS: 193149-74-5
MF: C20H15N6NaO11S2
MW: 601.48
Purity: Min99%
Appearance: Brown to white solid powder
Appearance: Brown to white solid powder
2. Primary Application Areas
Uses A highly sensitive tetrazolium reagent that produces a water-soluble formazan (orange color that absorbs at 460 nm) in NADH.
It is newer generation formazan-based dyes which release the converted prod uct into the medium in a soluble form allowing for a non-destructive determination of viability enabling the cells to be subject to further investigations.
Uses WST-8 is a next generation tetrazolium reagent that serves as a sensitive chromogenic indicator for NADH.
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