Tetrazolium Blue Chloride belongs to the class of tetrazolium salts and is a commonly used reagent for detecting cell viability in biochemistry.
- Basic information
- English name: Tetrazolium Blue Chloride
- CAS number: 1871-22-3
- Molecular formula: C40H32Cl2N8O2
- Molecular weight: 727.64
- Related categories: Fine chemicals; Dyeing agents and dyes
- Transport Information: Not dangerous goods
- Application
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Cell Viability Testing
It serves as an alternative to the MTT assay, where it is reduced by mitochondrial activity in living cells to form a deep purple formazan, enabling quantitative measurement of cell proliferation and cytotoxicity.
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Microbial Detection
Used as a chromogenic substrate for bacterial dehydrogenase activity assays, indicating microbial metabolic activity in applications such as water quality monitoring and food safety testing.
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