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Synonyms: N-[2-HYDROXY-1,1-BIS(HYDROXYMETHYL)ETHYL]TAURINE;2-[TRIS(HYDROXYMETHYL)METHYL]-2-AMINOETHANE SULFONIC ACID;2-[TRIS(HYDROXYMETHYL)METHYLAMINO]-1-ETHANESULFONIC ACID;TES free acid MF: C6H15NO6S MW: 229.25 Purity: 99% Product manager Email: Joy@coreychem.com
| Melting point | ~225 °C (dec.) |
| density | 1.260 (estimate) |
| refractive index | 1.5500 (estimate) |
| storage temp. | Keep in dark place,Sealed in dry,Room Temperature |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| form | Crystalline Powder |
| color | White |
| PH Range | 6.8 – 8.2 |
| Odor | Odorless |
| Water solubility | SOLUBLE |
| Product manager | Joy Wu; Email:Joy@coreychem.com |
Frequently Asked Questions Question 1: What are the main product characteristics of TES buffer? TES is a zwitterionic Good’s buffer. Its molecular formula is C₆H₁₅NO₆S. It appears as white crystalline powder, with good water‑solubility and negligible solubility in non‑polar organic solvents. Its pKa value is around 7.5 at 25 °C. It is highly soluble in water (about 70 g/100 mL at 20 °C, or ≥50 mg/mL at 25 °C) and is nearly insoluble in organic solvents. Question 2:What are the key features of TES compared with traditional buffers like phosphate? TES exhibits low metal‑ion chelating capacity, does not easily form precipitates with many metal cations. It shows minimal light absorption in the ultraviolet region commonly used for biological detection, and its pH changes slightly with temperature variation. A major advantage of TES is that, unlike Tris, it does not chelate or precipitate metal ions such as Mg²⁺ and Ca²⁺, making it suitable for experiments requiring divalent metals Question 3:What advantages does TES have for biological experimental applications? It has low toxicity to cells and enzymes, maintains stable pH in physiological pH range, seldom interferes with biochemical reactions. It resists microbial degradation better than some other Good’s buffers under normal working conditions. It serves in molecular biology for nucleic acid (DNA/RNA) electrophoresis, hybridization, and sequencing reactions, as well as in restriction digests, ligation, and PCR. Question 4: What should be noted when preparing TES aqueous solution? And What storage and handling conditions are recommended? Dissolve TES powder in purified water first, then adjust pH with dilute NaOH or HCl. Do not heat excessively during dissolving. Prepare working solution freshly for long‑term cell experiments to avoid contamination. TES should be stored sealed in a cool, dry place at room temperature (2–30 °C), protected from light and moisture, and kept away from strong oxidizing agents and high temperatures. It is generally regarded as a low-toxicity reagent, though environmental caution (especially regarding water bodies) is noted. Question 5: How is the pH‑buffering performance of TES affected by temperature? Its pKa decreases as temperature rises. The temperature coefficient is approximately −0.020 pKa/°C. It is necessary to measure and adjust pH at the actual experimental temperature instead of only at room temperature.
Application 1: What is the primary role of TES in cell and tissue culture?
TES serves as a buffering component in cell culture media, maintaining stable pH in in vitro environments—particularly for mammalian cells and embryonic stem cell culture.
Because TES does not chelate or precipitate divalent metal ions such as Mg²⁺ and Ca²⁺, it is well-suited for metal-ion-containing cell culture systems where other buffers would form precipitates.
It is also used as a cell wash buffer and in solutions for DNA precipitation and extraction.
Application 2:How is TES used in molecular biology applications?
In molecular biology, TES is used as a buffering system for nucleic acid (DNA/RNA) electrophoresis, hybridization, and sequencing reactions.
It is also a component of buffers for restriction enzyme digestion, ligation, and PCR. TES is particularly compatible with restriction enzyme assays—it affects restriction enzyme kinetics more than HEPES but less than Tris.
Application 3: What are the applications of TES in protein research and enzyme studies?
TES is used in protein extraction, purification, crystallization, and activity determination. It provides stable pH conditions for enzyme kinetics studies, helping to preserve enzyme activity.
In chromatography, TES is applied in gel filtration and affinity chromatography for protein separation. It is also used for enzyme activity assays, such as succinate oxidation measurements.
Application 4: How is TES applied in analytical and diagnostic (IVD) applications?
TES is suitable for use in the bicinchoninic acid (BCA) protein assay. It is applied in micellar electrokinetic chromatography and in Test Yolk Buffer medium.
In the in vitro diagnostics (IVD) field, TES is used as a raw material in diagnostic reagent kits, including biochemical diagnostic kits, DNA/RNA extraction kits, and PCR diagnostic kits.
Application 5:What are the less obvious or specialized applications of TES?
TES is used in cell lysis buffers to maintain isotonic sucrose concentrations within Percoll gradients during subcellular fractionation.
It also finds use in personal care and cosmetics, where it can protect active ingredients as a pH-regulating buffer. Additionally, TES is capable of influencing mRNA expression and embryo development
in certain biological studies, and it forms complexes with DNA and Cu(II) ions—properties that may be relevant in specialized experimental contexts.
Conventional packaging: 100G,1KG.5KG,10KG,25KG in sealed bottle or fiber can;Packaging according to customer requirements. Storage Conditions: Please store in a dry&cool,dark, inert environment at room temperature. For further information about the product, please contact: Product Manager Email: Joy@coreychem.com
