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Synonyms: DMT-2'O-ME-RU AMIDITE ;2'-O-methyluridine-ce phosphoramidite;2’-O-Methy-rUPhosphoramidite;N-blocked-5'-O-DMT-2'-O-Me CED uridine phosphoramidite; 5'-O-(4,4-Dimethoxytrityl)-2'-O-methyluridine-3'-(2-cyanoethyl-N,N-diisopropyl)phosphoramidite MF: C40H49N4O9P MW: 760.82 Product Manager Email: Joy@coreychem.com
| storage temp. | Sealed in dry,2-8°C |
| solubility | DMSO : 100 mg/mL (131.44 mM; Need ultrasonic) |
| form | powder |
| pka | 9.39±0.10(Predicted) |
| color | white to off-white |
| biological source | non-animal source (no BSE/TSE risk) |
| Purity | 98% |
| Product manager Email | Joy@coreychem.com |
Frequently Asked Questions
Question 1: What is the recommended storage condition for DMT-2′-O-Me-U Phosphoramidite CAS-no-110764-79-9?
The product should be stored sealed in dry conditions at 2–8°C. Some suppliers also recommend storage under inert gas and protection from light.
For long-term storage, it is stable for 3 years at -20°C and 2 years at 4°C in powder form.
Question 2:What are the key advantages of using this monomer? Enhanced nuclease resistance and improved target-binding affinity due to the 2'-O-methyl modification High yield of crude oligonucleotides Compatibility with standard DNA/RNA synthesis, allowing mixmer oligonucleotide production High purity (≥98–99% by HPLC/31P-NMR) and good chemical stability Stable coupling activity and good solubility in common organic solvents
Question 3: What is the recommended coupling time when used in synthesis? Synthesis of 2'-O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time: approximately 6 minutes is recommended, compared to 90 seconds for DNA monomers. Question 4: For what specific applications is DMT-2'-O-Methyl-rU Phosphoramidite commonly used? This compound is a key building block for synthesizing various modified oligonucleotides with therapeutic and diagnostic applications. Its primary uses include the synthesis of antisense oligonucleotides, siRNA (small interfering RNA), and miRNA mimics. The enhanced stability and binding affinity provided by the 2'-O-methyl modification make these oligonucleotides more effective in gene silencing and target binding. Additionally, it is used to create diagnostic probes and in fundamental molecular biology research where stable and specific hybridization is critical.
Application 1:What are the primary therapeutic applications of oligonucleotides synthesized using DMT-2'-O-Methyl-rU Phosphoramidite? This monomer is a key building block for synthesizing various therapeutic oligonucleotides. Its primary applications include the development of antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and aptamers. The 2'-O-methyl modification enhances nuclease resistance and improves pharmacokinetic properties, making these therapeutics more stable and effective in biological systems. For example, 2'-O-methyl-modified ASOs have been studied in clinical trials for inducing exon skipping in Duchenne muscular dystrophy, demonstrating good tolerability. Application 2:How is this compound utilized in the development of siRNA therapeutics specifically? In siRNA development, the 2'-O-methyl modification is strategically introduced to enhance stability in serum without significantly compromising RNA interference activity. Unmodified siRNA molecules are easily degraded in the body, but incorporating 2'-O-methyl uridine at specific positions protects them against serum-derived nucleases. This modification also helps ameliorate off-target toxicity and selectively protects the vulnerable 5'-end of the guide strand against exonucleolytic degradation in human blood serum. Rational design strategies often involve optimizing the positions of 2'-O-methyl modifications to maximize both stability and knockdown potency.
Application 3:What role does it play in diagnostic applications? In molecular diagnostics, 2'-O-methylated RNA monomers are used to create: Highly specific hybridization probes for nucleic acid detection Locked nucleic acid (LNA)-like probes that resist degradation in complex biological samples PCR and sequencing primers with improved thermal stability The enhanced duplex stability and nuclease resistance make these probes ideal for clinical diagnostics, including pathogen detection and gene expression analysis.
Application 4: How is it applied in antisense oligonucleotide (ASO) manufacturing?
In ASO development, the monomer is used to prepare fully or partially 2′-O-methylated antisense strands. The key benefits include:
Increased duplex stability with target mRNA
Enhanced RNase H-independent gene silencing when combined with other modifications
Better cellular uptake and reduced toxicity compared to phosphorothioate-only ASOs
It is commonly incorporated in gapmer designs — where a central DNA region is flanked by 2′-O-methyl RNA segments — to optimize both efficacy and safety.
Application 5:What are the advantages of this compound over standard DNA phosphoramidites in research applications? Compared to standard DNA phosphoramidites, the 2'-O-methyl version offers: Higher resistance to exo- and endonucleases — crucial for in vivo experiments Increased melting temperature (Tm) of resulting duplexes Compatibility with standard DNA/RNA synthesizers without needing special hardware Suitability for mixed DNA/RNA chimeric oligos in gene editing and functional studies
Packaging: 10G,50G,100G,1kg in sealed bottle or Bag;5kg,10kg,20kg,25kg in Fiber Can; Packing according to requirements. Transportation: Shipped by courier ( FEDEX,DHL), by Air flight. The forwarder is our long-term cooperated agent with professional experience for intermational transportation. We also could assistant with customs clearance and documents. Storage Conditions: Stored sealed in dry conditions at 2–8°C. For long-term storage,please keep at -20℃. For further information about the product, please contact: Product Manager Email: Joy@coreychem.com.
