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Yellow solid MF: C16H16ClN5 MW: 313.79
Synonyms: 2-(4-AMIDINOPHENYL)-6-INDOLECARBAMIDINEDIHYDROCHLORIDE;CELLSTAIN- DAPI SOLUTION;2-phenylindole-4′,6-dicarboxamidine dihydrohydrochloride (hydrate); 4′,6-DIAMINO-2-PHENYLINDOLE DIHYDROCHLORIDE;DAPI, DIHYDROCHLORIDE;DAPI
Product Categories: antibiotic;Indoles and derivatives;Fluorescent Labels & Indicators;
| Melting point | >300°C (dec.) |
| storage temp. | 2-8°C |
| solubility | H2O: 10 mg/mL heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks. |
| form | powder |
| color | Clear |
| PH | 4.0-5.0 (10g/l, H2O, 20℃) |
| Appearance | yellow solid or solution |
| Water Solubility | water: soluble |
| ε(extinction coefficient) | 30 at 263nm in H2O at 1mM |
| Stability: | PROTECT FROM LIGHT! Stable for 2 years as supplied. Solutions in DMSO may be aliquoted and stored at -20°C for up to 3 months. |
| Biological Applications | Nuclear apoptosis assay; targeting drug to cerebral neuron; treating amyloidosis,neurodegenerative diseases,Alzheimer’s disease,Down’s syndrome,type II diabetes; monitoring bioaerosols |
| Purity | 99% |
| Product manager | Joy Wu; Email: Joy@coreychem.com |
DAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications. It forms a fluorescent complex by attaching in the minor groove of A-T rich sequences of DNA. DAPI is often used as a counterstain, as its ultraviolet (max 358 nm) excitation and blue (max 461 nm) emission wavelengths separate it nicely from many popular primary fluorophores. It can be used on either fixed or live cells, although its low permeability in live cells demands that higher concentrations be used.
DAPI is used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex and as a cell permeable fluorescent minor groove-binding probe for DNA. For staining DNA in agarose gels.
DAPI has been used in rapid monitoring of microbial contamination,chromosomal banding technique, detection of apoptotic cells, fluorescence microscopy to track the DisA (DNA integritiy scanning protein) movement on Bacillus subtilis DNA to stain mature pollen grains.
CONTACT/Email address: Joy@coreychem.com
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