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Slightly yellow solid Powder MF: C46H46N2O23 MW: 994.86
Synonyms: 3′,6′-Di(O-acetyl)-2′,7′-bis[N,N-bis(carboxymethyl)aminomethyl]fluoresceintetraacetoxymethylester,DMSOsolution;Calcein AM solution;CALCEIN, AM;CAL-AM;4′,5′-Bis(N,N-bis(carboxymethyl)aminomethyl)fluorescein acetoxymethyl ester;Glycine, N,N’-((3′,6′-bis(acetyloxy)-3-oxospiro(isobenzofuran-1(3H),9′-(9H)xanthene)-2′,7′-diyl)bis(methylene))bis(N-(2-((acetyloxy)methoxy)-2-oxoethyl)-, bis((acetyloxy)methyl) ester;Calcein AcetoxyMethyl Ester;N,N’-[[3′,6′-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-2′,7′-diyl]bis(Methylene)]bis[N-[2-[(acetyloxy)Methoxy]-2-oxoethyl]glycine 1,1′-Bis[(acetyloxy) Methyl] Ester;
Product Categories: Amines;Aromatics;Fluorescent Labels & Indicators;Heterocycles;Intermediates & Fine Chemicals;Pharmaceuticals;CCalcium Signaling Research Tools;Calcium Probes;Fluorescent;Stains and Dyes;Xanthene
| Melting point | >250℃ |
| Boiling point | 982.7±65.0 °C(Predicted) |
| density | 1.49±0.1 g/cm3(Predicted) |
| refractive index | n20/D 1.479 |
| Fp | 85 °C |
| storage temp. | -20°C |
| solubility | DMSO: soluble10mg/mL, clear, colorless to slightly yellow |
| pka | 2.66±0.50(Predicted) |
| λmax | < 300 nm |
| Purity | 95% |
| Appearance | Slightly yellow solid Powder |
| Biological Applications | Calcium indicators; zinc indicators; cytotoxicity assays; apoptosis assays; labile iron pool assays; chemotaxis probes; cell adhesion probes; mitochondrial probes; P-glycoprotein probes; |
| Product manager | Joy Wu; Email: Joy@coreychem.com |
Calcein AM is a non-fluorescent, cell-permeable derivative of calcein, a self-quenching fluorescent dye. Calcein AM can be cleaved to release calcein in cells. Calcein is used as a substrate for multidrug resistance-associated protein 1 (MRP1) and multidrug resistance protein 3 (MDR3, P-glycoprotein 3). It is A colorless to slightly yellow solid. Soluble in dimethyl sulfoxide.
Calcein-AM appears to best satisfy the criteria for assaying cell adhesion and to have the least effect on cell viability and cell functions.
Calcein-AM is a hydrophobic non-fluorescent probe that can permeate the plasma membrane and can be hydrolyzed to Calcein (sc-202090), which is an extremely fluorescent and negatively charged molecule. Calcein-AM can be used to stain living cells and in cytoplasmic Schwann cells has been observed to fluoresce bright green. Calcein-AM has been reported to be a neutral substrate for P-glycoprotein (Pgp) and multidrug resistance protein (MRP) and has been used in flow cytometry studies to analyze the function of P-gp and MRP. A non-fluorescent, membrane permeable probe that can be hydrolyzed to a fluorescent molecule.
CONTACT/Email address: Joy@coreychem.com
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