Phenazine methosulfate Cas-no-299-11-6_News

Basic Overview and Fundamental Physicochemical Properties:
Phenazine methosulfate (PMS), officially registered under CAS No.299-11-6, is a synthetic heterocyclic aromatic organic compound with multiple aliases, including 5-Methylphenazinium methyl sulfate and Phenazine methyl sulfate. 
With a precise molecular formula of C₁₄H₁₄N₂O₄S and a standard molecular weight of 306.34 g/mol, this chemical compound presents itself as a bright orange to orange-red crystalline powder at room temperature and standard atmospheric pressure. 
As a vital and high-performance biochemical mediator, PMS has long been recognized as a staple reagent in biochemistry, cell biology, molecular biology, and biomedical analytical research. 

Core Biochemical Advantages:
-- High-Efficiency and Reversible Electron Transfer Capacity
First and foremost, the compound delivers ultra-efficient and reversible electron transfer capability, which is its most core competitive advantage in biochemical reaction systems. 
PMS acts as a rapid intermediate electron shuttle carrier between reduced coenzymes (NADH and NADPH) and chromogenic tetrazolium salts, such as MTT, XTT, and WST series dyes. 
In complex enzymatic redox reactions, ordinary electron mediators often suffer from slow electron transmission speed, incomplete reaction coupling, and low conversion efficiency.
In contrast, PMS can instantly capture electrons released by NADH/NADPH oxidation and efficiently transfer these electrons to tetrazolium substrates, triggering a thorough and stable chromogenic reduction reaction. 
--Excellent Chemical and Biochemical Stability
Secondly, PMS features exceptional chemical and biochemical stability under diverse experimental conditions. High-purity 98% PMS has excellent structural stability, resisting spontaneous oxidation, hydrolysis, and molecular degradation.
It maintains stable redox activity within temperature range of 4 °C to 37 °C, fully covering the temperature requirements of cell culture, enzymatic reaction incubation, and routine biochemical detection. 
Moreover, PMS shows strong tolerance to mild changes in pH values, remaining functionally stable in pH environments from 6.0 to 8.5, eliminating the need for strict and extreme reaction condition control. 
PMS participates in redox reactions with high specificity and produces no redundant interfering substances, effectively reducing experimental background noise and greatly improving the repeatability and credibility of batch experimental data.  
--Superior Solubility and Biological Compatibility
Thirdly, PMS owns excellent solubility and operational compatibility. The compound is highly soluble in distilled water, phosphate buffered saline (PBS), Tris-HCl buffer, and other common biological aqueous solutions, 
and also shows good solubility in low-polarity organic solvents. It can be quickly dissolved to form a clear and transparent homogeneous solution without precipitation or turbidity, even at conventional working concentrations used in biochemical detection. 
In addition, PMS has excellent biological compatibility with various biological samples, including cell culture supernatants, tissue homogenates, serum samples, and microbial fermentation broths.
Main Scientific and Industrial Research Applications:
--Biochemical Enzyme Activity Detection and Quantitative Analysis:
Beyond its prominent performance advantages, PMS (CAS 299-11-6) possesses irreplaceable and extensive application scenarios in life science research and biochemical analytical testing. 
Its most mainstream application lies in colorimetric-based biochemical enzyme activity detection and biomolecular quantitative analysis.
--Cell Viability Assay, Proliferation and Cytotoxicity Research
In cell biology and biomedical research, PMS is a critical reagent for cell viability detection, cell proliferation analysis, and cytotoxicity evaluation. 
The classic MTT-PMS and WST-PMS detection systems have become the most mainstream technical methods for in vitro cell experiments. In drug screening research, researchers utilize PMS-assisted chromogenic assays to evaluate 
the inhibitory or proliferative effects of new synthetic drugs, natural active ingredients, and chemical compounds on tumor cells, normal somatic cells, and microbial cells.
--Microbial Metabolism Research and Fermentation Engineering
Furthermore, PMS plays an important role in microbiological detection and metabolic mechanism research. It can be used to detect the metabolic activity and proliferation status of bacteria, fungi, and other microorganisms.
In microbial fermentation engineering research, PMS-coupled chromogenic systems monitor the activity of microbial metabolic enzymes in real time, helping researchers optimize fermentation conditions, improve strain activity, and enhance the yield of microbial metabolites. 
In addition, PMS is applied in construction of in vitro bionic redox reaction models, which are essential for studying the electron transfer mechanism of biological respiratory chains and exploring the energy metabolism rules of organisms at the molecular level.

Conclusion:
Phenazine methosulfate stands out among numerous biochemical mediators by virtue of its high-efficiency reversible electron transfer performance, superior environmental stability, excellent biological compatibility, and convenient operational characteristics.
It not only optimizes the sensitivity, accuracy and repeatability of traditional biochemical detection methods, but also provides reliable technical support for innovative research in cell biology, biomedicine, microbiology and metabolic biology. 
Product manager: Joy Wu         Email address:  Joy@coreychem.com