| Product name | O-Benzotriazole-N,N,N’,N’-tetraMethyl-uroniuM-hexafluorophosphate |
| Synonym | HBTU O-(1H-BENZOTRIAZOL-1-YL)-N,N,N’,N’-TETRAMETHYLURONIUMHEXAFLUOROPHOSPHATE; O-(BENZOTRIAZOL-1-YL)-N,N,N’,N’-TETRAMETHYLURONIUMHEXAFLUOROPHOSPHATE; O-BENZOTRIAZOL-1-YL-N,N,N’,N’-TETRAMETHYLURONIUMHEXAFLUOROPHOSPHATEPOLYMERBOUND; O-BENZOTRIAZOL-1-YL-TETRAMETHYLURONIUMHEXAFLUOROPHOSPHATE; O-BENZOTRIAZOLE-1-YL-N,N,N’,N’-TETRAMETHYLURONIUMHEXAFLUOROPHOSPHATE; O-BENZOTRIAZOLE-N,N,N’,N’-TETRAMETHYL-URONIUM; O-BENZOTRIAZOLE-N,N,N’,N’-TETRAMETHYL-URONIUM-HEXAFLUORO-PHOSPHATE |
| CAS NO. | 94790-37-1 |
| Appearance | Off-white to white crystalline powder |
| Purity | 99% Min |
| MF | C11H16F6N5OP |
| MW | 379.25 |
| Structural formula |  |
| Related categories | Coupling reagent |
| Transport Information | Non-hazardous materials |
| HS Code | 2933990099 |
| Product Manager Email | sophia@coreychem.com |
I. Core Applications (Most Mainstream in Peptide Synthesis Field)
1. Fmoc Solid-Phase Peptide Synthesis (SPPS)
Suitable for liquid-phase coupling reactions of short peptides, cyclic peptides, and modified peptides, it has strong compatibility and can adapt to various protecting groups and common reaction solvents (such as DMF, DCM, etc.), meeting the synthesis needs of peptides with different structures and being widely used in peptide research and development as well as small-batch production.
II. Extended Applications (Organic Synthesis/Medicinal Chemistry Field)
1. General Amide Bond Construction
It is mainly used for the condensation reaction of carboxylic acids and amines to efficiently construct amide bonds, covering scenarios such as small-molecule drugs, pharmaceutical intermediates, and total synthesis of natural products. For example, it can be used in the synthesis of heterocyclic compounds and complex molecular structures such as benzimidazoles, quinoxalines, glycoconjugates, and β-lactams, and it is one of the commonly used reagents for constructing amide bonds in organic synthesis.
2. Esterification Reaction
It can catalyze the esterification reaction between carboxylic acids and alcohols to generate target ester products, which is suitable for the synthesis of nucleotides, sugar esters, functional organic molecules, etc., and has wide applications in the fields of fine chemicals and biochemistry.
3. Other Condensation/Cyclization Reactions
It can convert carboxylic acids into heterocyclic compounds such as benzimidazoles and quinoxalines through a one-pot method; it can also be used for the cyclization reactions of cyclic peptides and lactams, simplifying reaction steps, improving reaction efficiency, and adapting to the synthesis needs of various complex molecules.
III. Product Advantages (For Application Reference)
HBTU is moderately priced, lower than the similar uronium salt coupling reagent HATU, and higher than the combination system of EDCI and HOBt; it is easy to operate as a single-component feeding reagent, without the need to weigh two reagents separately like the EDCI+HOBt combination, which can save operation time and reduce weighing errors; it has an extremely low degree of racemization, better than the EDCI+HOBt combination and slightly inferior to HATU; the reaction rate is fast, more efficient than the EDCI+HOBt combination, and the by-products HOBt and TMU are easy to remove, which does not affect the purity of the target product.
IV. Typical Reaction Conditions (Reference)
Feeding ratio: Carboxylic acid: Amine: HBTU: HOBt: DIPEA = 1:1.2:1.2:1.2:2.4; the reaction solvent can be DMF, DCM or a mixed solvent of the two; the reaction temperature can be controlled at room temperature (20–25℃) without high or low temperature conditions; the reaction time is 0.5–2 hours, and the reaction progress can be monitored by TLC (Thin Layer Chromatography) or UPLC (Ultra Performance Liquid Chromatography) to ensure complete reaction.
V. Summary of Application Scenarios
HBTU is mainly used in peptide research and development and production, covering the preparation of solid-phase/liquid-phase peptide synthesis, API intermediates, modified peptides, and cyclic peptides; in the field of medicinal chemistry, it is used for the synthesis of small-molecule amides, heterocycles, and ester drugs; in the field of organic synthesis, it can be used for the coupling reactions of natural products, functional molecules, and sugar/nucleotide conjugates, adapting to various scenarios such as scientific research, pilot test, and pilot-scale production.
Contact information:
Contact: Sophia Li
Email: sophia@coreychem.com
