N1-[2-((1S)-1-[(3-aminopropyl)amino]-4-[di(3-amino-propyl)amino]butylcarboxamido)ethyl]-3,4-di[oleyloxy]-benzamide(MVL5)

CAS: 464926-03-2

Formula Weight: 1164.862

Molecular Formula: C₅₉H₁₁₆Cl₅N₇O₄

Purity: >95%

Storage Temperature: -20°C

Product Mananger: selina@coreychem.com

Function of MVL5 (CAS: 464926-03-2)

MVL5 (Multivalent Lipid 5) is a multivalent cationic lipid primarily used in scientific research for nucleic acid delivery and gene transfection. It is typically formulated with helper lipids to create liposomes, nanoparticles, or lipid complexes that efficiently transport DNA, siRNA, mRNA, and other nucleic acids into cells.

1. Core Functions

1.1 Key Cationic Lipid for Nucleic Acid Delivery

MVL5 contains multiple positive charge centers, enabling it to strongly bind negatively charged nucleic acids such as DNA, siRNA, mRNA, and oligonucleotides. This interaction allows MVL5 to:

• Protect nucleic acids from nuclease degradation

• Maintain nucleic acid stability during delivery

• Increase affinity to the cell membrane and facilitate cellular uptake

Its multivalent structure provides stronger nucleic-acid condensation capability than monovalent cationic lipids.

2. Main Research Applications

2.1 siRNA Delivery (Gene Silencing Studies)

MVL5 forms nanoscale complexes with siRNA, enabling efficient cytoplasmic delivery for:

• Gene silencing (knockdown)

• Gene regulation studies

• RNA interference (RNAi) mechanism research

2.2 DNA / Plasmid Transfection

When combined with helper lipids (e.g., DOPE), MVL5 forms liposomes for DNA or plasmid transfection, commonly used for:

• Protein expression studies

• Reporter gene assays (e.g., GFP, luciferase)

• Gene function validation

2.3 mRNA and Nucleic Acid Nanoparticle Development

Although not a standard clinical LNP component, MVL5 is widely used in research for:

• Screening cationic lipids in nanoparticle formulations

• Delivery mechanism studies

• Comparative evaluation of nucleic acid carriers

2.4 Studies on Cell Uptake and Endocytosis

Due to its strong interaction with membranes, MVL5 is also used for:

• Liposome fusion mechanism studies

• Antibody–lipid complex research

• Model studies on cell–membrane interactions

3. Advantages of MVL5

3.1 Strong Nucleic Acid Condensation Ability

Its multivalent cationic structure allows tight and efficient nucleic-acid binding.

3.2 High Transfection Efficiency

MVL5 often shows superior performance—especially in cell types that are traditionally hard to transfect.

3.3 Lower Cytotoxicity Compared to Some Traditional Cationic Lipids

The multivalent design reduces disruptive interactions with the cell membrane, resulting in:

• Higher cell viability

• Better suitability for sensitive cell lines

3.4 Usable in Both In Vitro and Certain In Vivo Studies

MVL5-based complexes can be applied to cell experiments and some animal studies

(Research Use Only).

4. Usage Limitation

• For research use only

• Not intended for clinical or therapeutic applications

• Should be stored at low temperatures (e.g., −20°C) to maintain stability

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Contact us for more information: selina@coreychem.com