| CAS Number | Salt Form | Water Solubility | Stability | Preferred Application Scenarios |
|---|---|---|---|---|
| 2002-44-0 | MIF-1 Free Base | Poor, requires DMSO for solubilization | Low | Micro-scale short-term in vitro enzyme activity, SPR biochemical detection; animal injection prohibited |
| 1207536-22-8 | MIF-1 Monoacetate | Excellent | Highest | Long-term n |
1. CAS 2002-44-0 MIF-1 Free Base
- Sequence: L-Pro-L-Leu-Gly-NH₂ (C-terminal tripeptide of oxytocin at positions 7–9, endogenous brain peptide MIF-1)
- Molecular Formula: C13H24N4O3 , Molecular Weight: 284.36
- Physicochemical Properties: Poor water solubility, slightly soluble in pure water, requires a small amount of DMSO for solubilization; relatively low stability; free from acetate interference, suitable for trace in vitro biochemical screening.
2. CAS 1207536-22-8 MIF-1 Monoacetate
- Sequence: Exactly the same as above, complexed with 1 molecule of acetic acid to form a salt.
- Molecular Formula: C15H28N4O5 , Molecular Weight: 344.41
- Physicochemical Properties: Significantly improved water solubility, optimal stability in buffer systems; suitable for long-term cell incubation, large-scale intraperitoneal injection in animals, and high-throughput drug screening.
General Structural Functional Domains (Identical for both versions)
- N-terminal Pro rigid scaffold: The pyrrolidine ring fixes the peptide conformation, adapting to the allosteric binding pocket of the dopamine D2 receptor; lacks a free amino terminus, providing resistance against aminopeptidase degradation.
- Leu hydrophobic side chain hinge: Regulates transmembrane transport capability, enabling complete penetration of the blood-brain barrier (BBB) for direct central nervous system efficacy.
- C-terminal Gly-NH₂ amide (Essential for activity): Amide modification determines the triple activities of receptor allosteric activation, opioid antagonism, and MSH inhibition; replacing it with a free carboxyl group reduces biological activity by over 90%.
- No Trp/Tyr/Met: Almost no photosensitive oxidative degradation; linear short tripeptide, no disulfide bonds, no cysteine.
Unified Storage Stability
- Compatible with pH 6.0–7.4 dilute acetate serum-free culture media; no need to protect from light.
- Retains 91%~93% intact peptide in serum-free buffer at 4℃ for 24 hours; 40%~42% remaining in mammalian serum at 37℃ for 4 hours.
- Lyophilized powder shelf life is 3 years at -20℃; aqueous solutions should be aliquoted and stored at -80℃, avoiding repeated freeze-thaw cycles.
- Stability Ranking: 1207536-22-8 Acetate > 2002-44-0 Free Base.
Main research applications
1. Dopamine D2 Receptor Allosteric Modulator High-Throughput Screening (Core Positive Control)
Primary striatal neuron calcium fluorescence, receptor SPR binding assays — screening small molecules for anti-Parkinson’s and anti-depressant effects; distinguishing pharmacodynamic differences between positive and negative allosteric ligands.
2. Melanin Pigmentation and Whitening In Vitro / Animal Pathology Models
Melanocyte tyrosinase activity assays, mouse UV-induced hyperpigmentation models — evaluating lead compounds for spot-lightening and melanin inhibition.
3. Opioid Receptor Antagonist, Morphine Addiction and Pain Mechanism Models
Rat hot-plate / tail-flick analgesia tests, construction of opioid tolerance and withdrawal behavioral models — screening for anti-addiction and analgesic adjuvant drugs.
4. Short Peptide C-Terminal Amidation Modification Structure-Activity Relationship (SAR) Reference Peptides
Free base / acetate salt pairing — quantifying the regulatory effects of C-terminal amidation and salt formation on water solubility, CNS penetration, and receptor activity.
Samples and further technical data can be provided upon request.
More details, just contact: Nicole@coreychem.com
