High Purity Tris(hydroxypropyl)phosphine (THPP) (CAS 4706-17-6): Highly Efficient Disulfide Bond Reducing Agent with Full Analysis
1、Product Overview
Tris(hydroxypropyl)phosphine (THPP) is a highly effective organophosphorus reducing agent that occupies an important position in the field of biochemical and pharmaceutical research. As a classic disulfide bond reducing agent, its greatest value lies in its ability to efficiently reduce disulfide bonds, including those present in biologically important cysteine derivatives. It remains stable under both acidic and alkaline conditions, a feature that many conventional reducing agents cannot achieve.
2、Basic Product Information
1）Product Name: Tris(hydroxypropyl)phosphine (THPP)
2）CAS NO.: 4706-17-6
3）Product Category: Reducing Agent
4）Molecular Weight: 208.24
5）Appearance: Colorless to light-yellow transparent liquid
6）Purity (P-31 NMR): 90% above, it can be customized according to your needs.
7）Reducing Power (Titration Test Method): ≥86%
8）Pack Sizes: 10g, 100g, 1kg, 10kg, 100kg
9）Product Manager Email: selina@coreychem.com
3、Frequently Asked Questions (FAQ)
1）What is the difference between THPP and conventional reducing agents such as DTT or TCEP?
Compared with DTT, THPP exhibits superior reducing power and does not contain thiol groups, avoiding the risk of introducing unwanted thiol-disulfide exchange reactions. Compared with TCEP, THPP offers good water solubility and can be used under both acidic and alkaline conditions. Its stability and reactivity make it a versatile choice for DNA sequencing and biosensor development.
2）In which applications is THPP most commonly used?
THPP is widely employed in DNA sequencing, where it efficiently reduces disulfide bonds. It is also used in the preparation of hydrogel beads for nucleotide sequencing, the development of biosensors for biological analysis, and the synthesis of phosphorus-containing antibacterial polymers.
3）What advantages does THPP offer in DNA sequencing?
In DNA sequencing, the efficient reduction of disulfide bonds is critical for achieving reliable results. THPP exhibits excellent reactivity towards disulfide bonds and remains stable under the conditions used in sequencing workflows, making it a preferred reagent for this application.
4）Can THPP be used in the preparation of functional materials?
Yes. THPP plays a significant role in the preparation of phosphorus-containing antibacterial polymers. It can also be utilized in the synthesis of hydrogel beads for nucleotide sequencing applications, demonstrating its versatility beyond traditional reducing agent roles.
5）What issues should THPP pay attention to when storing?
THPP should be stored in a sealed container under inert gas protection (such as nitrogen or argon), away from light and moisture. It is recommended to store it in a cool, dark place and avoid repeated opening of the lid to prevent moisture absorption and deterioration.
4、Application Cases
1）DNA Sequencing
THPP is widely employed in DNA sequencing due to its ability to reduce disulfide bonds efficiently. Its stability under both acidic and alkaline conditions ensures reliable performance across different sequencing protocols.
2）Hydrogel Beads Preparation
THPP can be utilized in the synthesis of hydrogel beads for nucleotide sequencing applications. Its reducing properties contribute to the formation of stable hydrogel structures suitable for sequencing workflows.
3）Biosensors
THPP is a key reagent for developing biosensors used in biological analysis. Its ability to reduce disulfide bonds enables the functionalization of sensor surfaces and the stabilization of biomolecules.
4）Phosphorus-containing Antibacterial Polymers
THPP plays a significant role in the preparation of antibacterial polymers containing phosphorus. The introduction of phosphorus-containing groups can enhance the antibacterial properties of the resulting materials.
5、Our Capabilities
1）Scale of Production
We possess mature production capabilities for THPP, capable of meeting kilogram-level demands. Our production process is designed to ensure consistent quality and reliable supply.
2）Product Purity
Our THPP boasts a high purity level of 98%, ensuring its effectiveness in various applications. The purity can be customized according to your needs.
3）Reducing Power
With a minimum reducing power of 86%, our THPP guarantees reliable performance and results.
6、Safety and Storage
1）Store in a cool, well-ventilated area and keep the container sealed;
2）Sensitive to light, moisture, and air, it is recommended to store under inert gas protection (such as nitrogen or argon);
3）Store away from light, recommended temperature below 15 °C (some suppliers suggest -20 °C);
4）Store separately from strong oxidants and strong alkalis.
7、Product Manager Information
Product Manager: Selina
Email: selina@coreychem.com
