AMINOPEPTIDASE M
AMINOPEPTIDASE M is an important proteolytic enzyme widely used in biochemical research, pharmaceutical development, and industrial production.
This enzyme can specifically hydrolyze the leucine residue at the N-terminus of peptide chains, playing a key role in protein metabolism and cellular function regulation.
This article will comprehensively introduce leucine aminopeptidase from the aspects of product characteristics, application fields, storage conditions, and usage precautions.
- Product: Aminopeptidase M
- CAS number: 9054-63-1
- Molecular formula: C12H24O2
- Source: Usually extracted and purified from pig kidneys or microorganism
- Enzyme activity: The typical activity range is between 10-20 U/mg (depending on the actual batch)
Product Manager Email: ada@coreychem.com
Product Features
- High specificity: mainly hydrolyzes leucine at the N-terminus of peptide chains, with weak hydrolytic activity towards other amino acid residues.
- Good stability: Maintain high stability under suitable pH and temperature conditions.
- Widely applicable: suitable for various biochemical and molecular biology experiments.
- Superior purity: Through a multi-step purification process, the product ensures high purity and low impurity content.
Main application areas
1. Biomedical research
Leucine aminopeptidase has important value in cell signal transduction, protein metabolism research, and exploration of disease mechanisms. Especially in tumor research, the expression level of this enzyme is often used as a biomarker for certain cancers.
2. Drug development
As an important tool for drug metabolism research, enzymes are used to evaluate the stability and metabolic pathways of peptide drugs. During the drug design phase, it can be used to study the effectiveness of enzyme inhibitors.
3. Industrial production
Used for protein hydrolysis and flavor improvement in the food industry; Used in the cosmetics industry to prepare active peptide raw materials.
4. Diagnostic reagents
As a component of clinical diagnostic kits, it is used to detect biomarkers of related diseases.
Usage recommendations
- Working concentration: Depending on specific experimental requirements, a concentration of 0.1-1.0 U/mL is usually used.
- Reaction buffer: Tris HCl or phosphate buffer (pH 7.0-7.5) is recommended.
- Reaction time: usually 30 minutes to 2 hours, adjusted according to substrate concentration and enzyme quantity.
Storage and stability
- Storage conditions: Dry and store at -20 ° C to avoid repeated freezing and thawing.
- Dissolving method: Dissolve gently in deionized water or appropriate buffer solution, avoiding vigorous shaking.
- Shelf life: Under correct storage conditions, it can maintain activity for at least 12 months.
If you would like to obtain a quotation for this product, please contact: ada@coreychem.com
