📌Basic Information
| Product name | 2′,7′-Dichlorodihydrofluorescein Diacetate |
| Synonym | 187263;2,7-Dichlorofluorescin Diacetate – CAS 4091-99-0 – Calbiochem;2,7-Dichlorodihydrofluorescein diacetate (2,7-Dichlorofluorescin diacetate |
| CAS NO. | 4091-99-0 |
| Appearance | White solid |
| Content | ≥99.0% |
| MF | C24H16Cl2O7 |
| MW | 487.29 |
| Related Categories | Fluorescent probes, labeling, particles and staining; Biochemical reagents – plant hormones and nucleic acids |
| Transport Information | no dangerous goods |
| HS Code | 3204200000 |
| Application | Fluorescent probe |
| Packaging | 50mg/bag；1g/bag；100g/bag |
| Contact Info | lucy@coreychem.com |
📋 Basic Information
Chinese Name: 2′,7′-Dichlorodihydrofluorescein diacetate (Common synonym: H₂DCFDA)
Molecular Formula/Molecular Weight: C₂₄H₁₆Cl₂O₇ / 487.29 g/mol
Appearance: Off-white to white solid powder
Purity: Commercially available products are typically >99%
🔬 Detection Principle (Three-Step Cascade Reaction)
This is the core mechanism by which the probe functions; understanding it will help you design your experiments more effectively:
Entry into the cell (permeability): DCFH-DA is uncharged and lipophilic, allowing it to freely pass through the cell membrane of living cells and enter the cytoplasm.
Trapped Inside the Cell (Deacetylation): Once inside the cell, intracellular esterases hydrolyze the diacetate group on the probe, converting it into DCFH (dichlorodihydrofluorescein), a charged compound that cannot cross the cell membrane. This effectively “locks” the probe inside the cell.
Fluorescence activation (oxidation): In the presence of reactive oxygen species (such as hydrogen peroxide or hydroxyl radicals), the non-fluorescent DCFH is rapidly oxidized and converted into DCF (dichlorofluorescein), which emits strong green fluorescence.
Summary: The intensity of fluorescence is directly proportional to the level of reactive oxygen species within the cell. By detecting the fluorescence of DCF, the oxidative stress status of cells can be quantitatively or qualitatively analyzed.
✨ Key Advantages and Features
High sensitivity and low background: The probe itself is non-fluorescent and only emits light after reacting with ROS, ensuring an extremely high signal-to-noise ratio.
High cellular permeability: Efficiently enters live cells without the need for special transfection reagents, making it easy to use.
Real-time detection: Enables dynamic, real-time monitoring within live cells to observe changes in ROS levels over time.
Excellent Compatibility: The fluorescence spectrum of the product, DCF, is very similar to that of FITC (fluorescein isothiocyanate). It can be detected using standard FITC channels with 488 nm excitation and 525 nm emission, making it suitable for a variety of instruments, including fluorescence microscopes, flow cytometers, and fluorescence microplate readers.
⚙️ Core Applications
Oxidative Stress Detection: Widely used to assess changes in total cellular reactive oxygen species (ROS) levels induced by drugs, environmental pollutants, or physical stimuli (such as UV radiation).
Antioxidant Efficacy Evaluation: Screening and validating antioxidants by comparing the extent of ROS reduction following the addition of candidate compounds.
Cell Function Studies: Detecting changes in the baseline redox state of cells following specific gene knockout or overexpression.
Pathological Model Studies: Used to investigate disease models closely associated with oxidative stress, such as neurodegenerative diseases, inflammation, cardiovascular diseases, and cancer.
📧 Contact Info
lucy@coreychem.com
